Quantitative Proline Analysis by HPLC-QTOF

Quantitative analysis of proline was performed using a 1260 Infinity HPLC coupled to a 6530 Q-TOF mass spectrometer equipped with a dual ESI source (Agilent Technologies, Santa Clara, CA, United States) in the positive ionization mode according to the method of Klupczynska et al. (Klupczynska et al., 2020 (link)). Chromatographic separation was conducted on a Luna HILIC column (2.0 × 100 mm, 3 μm particle size, Phenomenex, Torrance, CA, United States). The total protein concentration was used for normalization. The data were presented as a percent of control values.

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Huynh T.Y., Oscilowska I., Szoka L., Piktel E., Baszanowska W., Bielawska K., Bucki R., Miltyk W, & Palka J. (2022). Metformin Induces PRODH/POX-Dependent Apoptosis in Breast Cancer Cells. Frontiers in Molecular Biosciences, 9, 869413.

Publication 2022

1260 Infinity HPLC 6530 Q-TOF mass spectrometer dual ESI source Luna HILIC column

Chromatographic Hplc Proline Protein

Corresponding Organization : Medical University of Białystok

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Quantitative analysis of proline

control variables

Total protein concentration (used for normalization)

controls

No positive or negative controls were explicitly mentioned

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