Migratory and Invasive Abilities of Cancer Cells

Example 3

Cell migration is a highly-integrated and multi-step process that plays an important role in the progression of late-stage cancer. Cell invasion is involved in extracellular matrix degradation and proteolysis. In the study, wound healing assay and transwell invasion assay were used to examine migratory and invasive abilities of PDV cells, respectively, with or without PLX4032 stimulation. In invasion assay, PLX4032 promoted the invasive ability of PDV cells (FIG. 3). Further, in the presence or absence of PLX4032, KWM-EO, LM-EO and L+C treatment for 24 h reduced invaded cells on concentration-dependence.

In wound healing assay, 50 μg/mL KWM-EO, 50 μg/mL LM-EO and 40 μg/mL L+C reduced PDV cell migratory ability at 24 h treatment, and LM-EO had a better effect than the others (FIG. 4). On the other hand, 2 μM PLX4032 treatment strongly promoted cell migration of PDV cells within 24 h treatment, KWM-EO, LM-EO and L+C combination, similarly both EOs and compounds only, significantly suppressed PLX4032-stimulated migratory ability of PDV cells.