Peptide fragments of wheat and barley AMP-like peptides were produced by solid-phase synthesis using Fmoc chemistry (Elabscience Biotechnology Inc., Wuhan, China). The synthesized peptides were purified by RP-HPLC. Their identity to the required sequences was proved by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric analysis on an Ultraflex MALDI-TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) in a linear or reflector positive ion mode using α-cyano-4-hydroxycinnamic acid as a matrix.
The following characteristics of the synthesized peptides were calculated using the ExPASy ProtParam tool [41 ]: molecular weight, pI, net charge at pH 7, GRAVY index and aliphatic index. Hydrophobic moment μH was calculated with HeliQuest [42 (link)]. The Boman index was computed using APD3 [43 (link)]. The prediction of antimicrobial properties was carried out with CAMPR3 [44 (link)].
The following characteristics of the synthesized peptides were calculated using the ExPASy ProtParam tool [41 ]: molecular weight, pI, net charge at pH 7, GRAVY index and aliphatic index. Hydrophobic moment μH was calculated with HeliQuest [42 (link)]. The Boman index was computed using APD3 [43 (link)]. The prediction of antimicrobial properties was carried out with CAMPR3 [44 (link)].
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