All embryos were cultured in sequential media (Cook Media) to the blastocyst
stage. Approximately 3-8 trophectoderm (TE) cells were aspirated from high and
medium grade embryos using a biopsy pipette (internal diameter, 30 mm) and
dissected with a Zilos TK laser (Hamilton Thorne, MA). Biopsied TE cells were
washed in GV HEPES medium (INGAMED) and PGT-A was performed using
next-generation sequencing (NGS) based on the method described by the Beijing
Genomics Institute (Tan et al.,
2014 (link)).
For NGS, the genetic material within embryonic cells was isolated and amplified.
DNA analysis was then performed via NGS to detect chromosome aneuploidies and
some segmental aneuploidies (missing or extra segments of chromosomes). NGS can
detect segments of chromosomes larger than 5 megabases (MB). NGS cannot
distinguish between normal versus balanced embryos. The tests may not detect all
forms of polyploidy, balanced structural chromosome abnormalities, or
alterations smaller than 5 MB or in a heterochromatic region.
All embryos were frozen after biopsy and transferred on subsequent cycles.
stage. Approximately 3-8 trophectoderm (TE) cells were aspirated from high and
medium grade embryos using a biopsy pipette (internal diameter, 30 mm) and
dissected with a Zilos TK laser (Hamilton Thorne, MA). Biopsied TE cells were
washed in GV HEPES medium (INGAMED) and PGT-A was performed using
next-generation sequencing (NGS) based on the method described by the Beijing
Genomics Institute (Tan et al.,
2014 (link)).
For NGS, the genetic material within embryonic cells was isolated and amplified.
DNA analysis was then performed via NGS to detect chromosome aneuploidies and
some segmental aneuploidies (missing or extra segments of chromosomes). NGS can
detect segments of chromosomes larger than 5 megabases (MB). NGS cannot
distinguish between normal versus balanced embryos. The tests may not detect all
forms of polyploidy, balanced structural chromosome abnormalities, or
alterations smaller than 5 MB or in a heterochromatic region.
All embryos were frozen after biopsy and transferred on subsequent cycles.
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