Odors were purchased at their highest available purity (>97%) and diluted to 1 Pa in light mineral oil (Sigma Aldrich; St. Louis, MO) prior to placing in the flange caps / odor ports and included L-carvone, decanol, geraniol, guaiacol, and thioglycolic acid (Sigma Aldrich; St. Louis, MO). Odors in their stock concentration and diluted concentration were each stored under nitrogen to maintain purity. The panel of odors selected was based upon our prior work (Jagetia et al., 2018 (link)), wherein we found that [at least in a different background strain of mice] these odors elicited the widest assortment of odor preferences, with decanol and thioglycolic acid being investigated the least and most, respectively.
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Olfactory Preferences Profiling Protocol
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Other organizations : University of Florida, Van Andel Institute
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Variable analysis
independent variables
- Odor type (L-carvone, decanol, geraniol, guaiacol, and thioglycolic acid)
- Odor preferences
- Purity of odors (>97%)
- Dilution of odors to 1 Pa in light mineral oil
- Storage of odors (in their stock and diluted concentrations) under nitrogen to maintain purity
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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