Pluripotent Stem Cell Culture Protocol

hESCs line WA09 (H9)^{2 (link)} was purchased from WiCell Research Institute (http://www.wicell.org) and hiPSCs line FN2.1 was previously derived from human foreskin fibroblasts^{54 (link)}. All methods were performed by the relevant guidelines and regulations. Ethical approval was received by the local Ethics Committee (Comité de ética en Investigaciones biomédicas del Instituto FLENI) and written informed consent was obtained from the donor before foreskin fibroblast isolation. hPSCs were cultured on Vitronectin (VTN-N, Gibco) (0.5 µg/cm²) coated dishes in combination with fully defined Essential 8 medium (E8, Gibco) to 80–90% confluency. All cell lines were free of Mycoplasma sp. infection, which was tested as previously described^{55 (link)}.