Kinetic Mechanism of hMAO Inhibition

The hMAO inhibition effect of the EM derivatives
was determined using the MAO–Glo kit (Promega, Madison, WI,
USA). All the experimental conditions and procedures used for this
chemiluminescent assay were similar to those reported in our previous
paper.^{67 (link)} The kinetic mechanism of enzyme
inhibition was identified via Lineweaver–Burk and Dixon plots.^{68 (link),69 (link)} Different concentrations of MAO substrates were used in the presence
or absence of the test compounds, and the procedure was similar to
the enzyme inhibition assay.

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Paudel P., Shrestha S., Park S.E., Seong S.H., Fauzi F.M., Jung H.A, & Choi J.S. (2020). Emodin Derivatives as Multi-Target-Directed Ligands Inhibiting Monoamine Oxidase and Antagonizing Vasopressin V1A Receptors. ACS Omega, 5(41), 26720-26731.

Publication 2020

MAO–Glo kit

Assay Enzyme assay Inhibition Kinetic Promega

Corresponding Organization : Jeonbuk National University

Other organizations : University of Mississippi, University of Adelaide, Universiti Teknologi MARA

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Variable analysis

independent variables

Concentrations of MAO substrates

dependent variables

HMAO inhibition effect of the EM derivatives

control variables

Experimental conditions and procedures used for the chemiluminescent assay were similar to those reported in the previous paper

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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