Animal experiments were carried out in compliance with the Association for Assessment and Accreditation of Laboratory Animal Care International (http://www.aaalac.org/index.cfm ), and protocols were approved by the Institutional Animal Care and Use Committees (IACUC) of Tongji University.
For limb amputation, animals were anesthetized in 1/10 MMR containing 0.02% MS-222 (from stock of 0.5% MS-222, Sigma-Aldrich, United States, pH 7.5 buffered with Tris-Cl), NF stage 52–53 tadpole hindlimbs were amputated with a surgical scissor at the level of presumptive knee, and froglet forelimbs were amputated at mid-ulna/radius (Zhang et al., 2018a (link)). For tail amputation, NF stage 49–51 tadpoles anesthetized were amputated with a surgical blade perpendicular to the notochord, removing 40% of the tail (Lin and Slack, 2008 (link)).
For limb amputation, animals were anesthetized in 1/10 MMR containing 0.02% MS-222 (from stock of 0.5% MS-222, Sigma-Aldrich, United States, pH 7.5 buffered with Tris-Cl), NF stage 52–53 tadpole hindlimbs were amputated with a surgical scissor at the level of presumptive knee, and froglet forelimbs were amputated at mid-ulna/radius (Zhang et al., 2018a (link)). For tail amputation, NF stage 49–51 tadpoles anesthetized were amputated with a surgical blade perpendicular to the notochord, removing 40% of the tail (Lin and Slack, 2008 (link)).
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