Transcriptome Analysis of Wheat-Fungus Interaction

For transcriptome analyses, mycelia were harvested by conidium cultivation in potato dextrose liquid medium at 25°C in darkness for 12 h. A pot culture experiment was used to harvest the infection samples. Sterile millet was inoculated with F. pseudograminearum mycelia, incubated at 25°C for 7 days, and then mixed with sterile soil (0.5% inoculation millet), in which wheat was subsequently grown. For negative controls, sterile millet was used. After 5 and 15 days, wheat roots from each pot were collected and washed thoroughly under running tap water and distilled water so that no soil particles remained. Samples of 6 μg of total RNA were extracted from each of the above-mentioned samples using the total RNA purification system (Invitrogen, Carlsbad, CA, USA). The total transcriptome was sequenced by the Gene Denovo Company (Guangzhou, China). Transcriptome raw data have been submitted and are publicly available in the NCBI database (accession number SUB5545839) (37 (link)).