The C protein gene was fused to the mVenus fluorescent protein gene, and tested for use in SMT technology (Supplementary Figures S4 and S5 of Supplementary Materials). The SMT setup we used is explained in (28 ,42 (link)). Briefly, the central part of a 514 nm laser beam was used for stream acquisition (20 ms integration time) of mVenus fusions, and movies were captured by a Hamamatsu ImageEM EMCCD camera (128×128 pixel area of chip used). About 160 W cm−2 were applied onto the image plane. Protein fusions were expressed at very low levels, such that the single molecule level was reached after few frames of bleaching of most molecules present in the cell, followed by single step bleaching of the fluorophores. Expressing very few molecules also avoids localization artefacts due to overproduction of the respective protein. The images were taken for an average of 8 time intervals, with about 10% of tracks being longer than 10 steps. Only tracks of 5 and more steps were included in the analyses. Data analyses were done using the SMTracker 2.0 program (43 (link)).