Antiviral Efficacy Testing of CH223191 and Kynurenine

Vero and Huh-7 cell monolayers were pretreated with CH223191 and kynurenine diluted in MEM 1.5% NBCS for 1 h or 24 h, respectively, at 37 °C. CH223191 was used at concentrations ranging between 1.25 and 20 µM, while kynurenine was used at concentrations ranging between 5 and 80 µM. Then, viral infection was performed with either the IV4454 or Candid#1 strain at an MOI of 0.5. After 1 h, the inocula were removed and the cells were exposed to both compounds for 48 h at the mentioned concentrations. At 48 h pi supernatants were collected for plaque assay and cell monolayers were processed for either indirect immunofluorescence or RT-qPCR.