Western blotting was performed according to the conventional method with minor modifications (17 (link)). Following antibodies were used; a polyclonal anti-BRCA1 antibody specific for residues 1528–1863 of BRCA1, anti-BRCA2 (sc-8326, Santa Cruz Biotechnology), anti-RAD51 (GTX100469, GeneTax), anti-ATF1 (HPA055069, Sigma-Aldrich), anti-α-tubulin (DM1A, Merck), anti-β-actin (6D1, Wako Purechemicals), anti-MBP (E8032, New England Biolabs).
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