Lumbar segment of spinal cord lysates prepared from adult Sprague Dawley rats were generated by homogenization and sonication in RIPA buffer (50 mM Tris-HCl, pH 7.4, 50 mM NaCl, 2 mM MgCl2, 1% (vol/vol) NP40, 0.5% (mass/vol) sodium deoxycholate, 0.1% (mass/vol) SDS). The RIPA buffer included freshly added protease inhibitors (Cat# B14002, Biotools, Houston, TX), phosphatase inhibitors (Cat# B15002, Biotools, Houston, TX), and Benzonase (Cat# 71206, Millipore, Billerica, MA). Protein concentrations were determined using the BCA protein assay (Cat# PI23225, Thermo scientific). Glutathione magnetic beads (Cat# B23702, Biotools, Houston, TX), pre-incubated with purified CRMP2-GST (~0.4 μM), were incubated overnight with 300 μg of total protein from spinal cord lysates at 4°C in the absence or presence of the indicated peptides with gentle rotation. Beads were washed 3 times with RIPA buffer before re-suspension in Laemmli buffer and denaturation (5 min at 95°C) and immunoblotting [12 (link); 42 (link); 90 (link)]. Immunoblots were revealed by enhanced luminescence (WBKLS0500, Millipore) before exposure to photographic film. Films were scanned, digitized, and quantified using Un-Scan-It gel version 6.1 scanning software (Silk Scientific Inc, Orem, UT).