For routine histology, 3D-sandwich culture inserts were fixed in 4% paraformaldehyde (30 min) and embedded in paraffin. The 5 µm thick sections were cut and stained as described previously [9 (link)]. Briefly, sections were stained with hematoxylin (Haematoxylin Gill III, Surgipath, Leica, Germany) for 2 min and 1% eosin Y (Surgipath, Leica, Germany) for 1 min to examine the gross architecture and epithelial stratification.
Immunostaining of paraffin sections of 3D-sandwich culture inserts was performed as previously described [46 (link)]. The list of antibodies is provided inSupplementary Table S2 .
Immunostaining of paraffin sections of 3D-sandwich culture inserts was performed as previously described [46 (link)]. The list of antibodies is provided in
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