Generation and Validation of Pancreatic Cell Lines

Cell lines (PANC-1, Capan-1, HS766T, MIA-PaCa2, and CFPAC) were purchased from ATCC (Manassas, VA). HuR knockout clones were generated as previously described (17 (link),18 (link)). PDX line SPC_145 was a gift from Dr. Talia Golan (Sheba Medical Center) (19 (link)). All cell lines were tested monthly for mycoplasma and every 6 months STR validated. Cell lines for all experiments were below passage 20.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Schultz C.W., McCarthy G.A., Nerwal T., Nevler A., DuHadaway J.B., McCoy M.D., Jiang W., Brown S.Z., Goetz A., Jain A., Calvert V.S., Vishwakarma V., Wang D., Preet R., Cassel J., Summer R., Shaghaghi H., Pommier Y., Baechler S.A., Pishvaian M.J., Golan T., Yeo C.J., Petricoin E.F., Prendergast G.C., Salvino J., Singh P.K., Dixon D.A, & Brody J.R. (2021). The FDA approved anthelmintic Pyrvinium Pamoate inhibits pancreatic cancer cells in nutrient depleted conditions by targeting the mitochondria. Molecular cancer therapeutics, 20(11), 2166-2176.

Publication 2021

PANC-1 Capan-1 HS766T MIA-PaCa2 CFPAC

Cell lines Clones Mycoplasma

Corresponding Organization : Oregon Health & Science University

Other organizations : Thomas Jefferson University, Lankenau Institute for Medical Research, Georgetown University, George Mason University, University of Kansas, University of Nebraska Medical Center, The Wistar Institute, NeuroDevelopment Center, University of Baltimore, Johns Hopkins University, Sheba Medical Center, Tel Aviv University

Top 5 similar protocols

Variable analysis

independent variables

Cell lines (PANC-1, Capan-1, HS766T, MIA-PaCa2, and CFPAC)
HuR knockout clones

dependent variables

Measured outcomes

control variables

Cell lines were tested monthly for mycoplasma and every 6 months STR validated
Cell lines for all experiments were below passage 20

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!