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If not stated otherwise, flies were kept at 25°C on either PF or YF under a 12hr light/12hr dark cycle. UAS:lrp1RNAi and UAS:lrp2RNAi lines are from Vienna Drosophila RNAi Center, and oregonRC, repo-Gal4, dilp2-Gal4, dilp2, UAS:gfpRNAi, UAS:GCaMP, UAS:trpA1 lines are from Bloomington Stock center. Published are UAS:ltpRNAi, ltp (Palm et al., 2012 (link)), imp-l2-Gal4 (Bader et al., 2013 (link)), lpp-Gal4 (Brankatschk and Eaton, 2010 (link)), lpr1, lpr2 (Khaliullina et al., 2009 (link)), lrp1, lrp2 (Riedel et al., 2011 (link)), moody-Gal4 (gift from C Klaembt), impl2-Gal4 (gift from E Hafen). UAS:cherry and rab3-Gal4 were generated by transforming VK37 or VK33 flies (Venken and Bellen, 2005 (link)) with our cloned constructs. Yrab3 and Yrab27 are N-terminally YFP^MYC tagged viable rab alleles (rab3 and rab27) generated by targeted YFP^MYC integration into the endogenous locus (publication in preparation).

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Brankatschk M., Dunst S., Nemetschke L, & Eaton S. (2014). Delivery of circulating lipoproteins to specific neurons in the Drosophila brain regulates systemic insulin signaling. eLife, 3, e02862.

Publication 2014

dilp2-Gal4 UAS:gfpRNAi UAS:trpA1

Alleles Cherry Drosophila Flies Palm Rnai

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Variable analysis

independent variables

Genotypes (e.g., UAS:lrp1RNAi, UAS:lrp2RNAi, oregonRC, repo-Gal4, dilp2-Gal4, dilp2, UAS:gfpRNAi, UAS:GCaMP, UAS:trpA1, UAS:ltpRNAi, ltp, imp-l2-Gal4, lpp-Gal4, lpr1, lpr2, lrp1, lrp2, moody-Gal4, impl2-Gal4, UAS:cherry, rab3-Gal4, Yrab3, Yrab27)

dependent variables

Not explicitly mentioned

control variables

Temperature (25°C)
Food type (PF or YF)
Light/dark cycle (12hr light/12hr dark)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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