For our experiments, a MRSA strain (strain ID: BfR 08S00974, ITU 1179) of the sequence type (ST) 398 and the spa type t011 was obtained from the “Federal Institute for Risk Assessment” (BfR) and used as previously reported [4 (link), 12 (link)]. Briefly, MRSA was streaked out on blood base agar plates (Blood Agar Base No. 2, Oxoid, Germany) and incubated for 8 h. Then, the plates were suspended with PBS, adjusted to McFarland 0.5 and diluted to the defined MRSA concentration. The suspension was split into portions of 50 mL and stored on ice until aerosolization.
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MRSA Strain Preparation for Aerosolization
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Other organizations : Freie Universität Berlin
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Variable analysis
independent variables
- MRSA strain (strain ID: BfR 08S00974, ITU 1179) of the sequence type (ST) 398 and the spa type t011
- MRSA concentration in the suspension
- Blood Agar Base No. 2 (Oxoid, Germany) for streaking out MRSA
- Incubation time of 8 h for the MRSA on the blood base agar plates
- PBS (Phosphate-Buffered Saline) for suspending the MRSA colonies
- McFarland 0.5 standard for adjusting the MRSA suspension
- 50 mL portions of the MRSA suspension for aerosolization
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