KO constructs using the dual gRNA approach41 (link) targeting ASIC1 exon 2 and 3, and donor for ASIC1 were described previously.5 (link) ASIC1 expression vector used for rescue experiments was constructed by cloning ASIC1-coding region into pCDH-Myc by Cold Fusion kit (System Biosciences, Mountain View, CA, USA). All PCR products were verified by DNA sequencing.
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