Comparative Lm Strain Typing: cgMLST vs. PFGE

To compare cgMLST with PFGE for Lm strain typing, we analysed, in parallel, 100 isolates (57 human isolates, 33 food isolates and 10 isolates from food production environments) prospectively collected between January and April 2015 in the frame of the French listeriosis surveillance system by the National Reference Center for Listeria (Institut Pasteur, France). PFGE restriction profiles were obtained using the enzymes AscI and ApaI according to PulseNet standardized procedures (http://www.cdc.gov/pulsenet/PDF/listeria-pfge-protocol-508c.pdf) and were analysed using BioNumerics. PFGE and cgMLST typing results were compared using Simpson’s index of diversity and the adjusted Wallace index of concordance (see Supplementary Section 2.5 for details).

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Moura A., Criscuolo A., Pouseele H., Maury M.M., Leclercq A., Tarr C., Björkman J.T., Dallman T., Reimer A., Enouf V., Larsonneur E., Carleton H., Bracq-Dieye H., Katz L.S., Jones L., Touchon M., Tourdjman M., Walker M., Stroika S., Cantinelli T., Chenal-Francisque V., Kucerova Z., Rocha E.P., Nadon C., Grant K., Nielsen E.M., Pot B., Gerner-Smidt P., Lecuit M, & Brisse S. (2016). Whole genome-based population biology and epidemiological surveillance of Listeria monocytogenes. Nature microbiology, 2, 16185.

Publication 2016

Apai Enzymes Food Frame Human Listeria Listeriosis Pfge Strain

Corresponding Organization :

Other organizations : Organisation Mondiale de la Santé Animale, Institut Pasteur, Centre National de la Recherche Scientifique, Centers for Disease Control and Prevention, Statens Serum Institut, Public Health England, Public Health Agency of Canada, Biology of Infection, Santé Publique France, Inserm

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Variable analysis

independent variables

CgMLST

dependent variables

Lm strain typing

control variables

100 isolates (57 human isolates, 33 food isolates and 10 isolates from food production environments) prospectively collected between January and April 2015 in the frame of the French listeriosis surveillance system by the National Reference Center for Listeria (Institut Pasteur, France)
PFGE restriction profiles obtained using the enzymes AscI and ApaI according to PulseNet standardized procedures

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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