DNMT3A R882H Mutation Analysis

PCR sequencing reaction was performed as previously described [22 (link)]. Amplified products were purified using the PCR Purification Kit (Qiagen) according to the manufactures instruction. Sequencing was performed using ABI310 Genetic Analyzer (Applied Biosystems), and data were analyzed using DNA Sequencing Analysis Software v.5.2.0. Endonuclease restriction analysis of DNMT3A R882H mutation was performed using Fnu4HI (New England Biolabs) as previously reported [22 (link)].

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Berenstein R., Blau I.W., Suckert N., Baldus C., Pezzutto A., Dörken B, & Blau O. (2015). Quantitative detection of DNMT3A R882H mutation in acute myeloid leukemia. Journal of Experimental & Clinical Cancer Research : CR, 34(1), 55.

Publication 2015

PCR Purification Kit ABI310 Genetic Analyzer Fnu4HI

Abi310 Dna sequencing analysis Mutation Restriction endonuclease analysis

Corresponding Organization :

Other organizations : Charité - Universitätsmedizin Berlin

Top 5 similar protocols

Variable analysis

independent variables

PCR sequencing reaction protocol
PCR Purification Kit (Qiagen)
ABI310 Genetic Analyzer (Applied Biosystems)
DNA Sequencing Analysis Software v.5.2.0
Fnu4HI (New England Biolabs) for endonuclease restriction analysis of DNMT3A R882H mutation

dependent variables

Sequencing data
Results of endonuclease restriction analysis of DNMT3A R882H mutation

control variables

Previously described PCR sequencing reaction protocol [22]
Previously reported endonuclease restriction analysis of DNMT3A R882H mutation [22]

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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