Bisulfite Conversion and PCR Amplification

Bisulfite-converted DNA was PCR-amplified using primers designed in the Pyromark Assay Design Software (Qiagen, Germantown, MD, USA), with details of the assay and quality control previously published [22 (link)]. PCR and sequencing primers are listed in Table S1.

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Yang I.V., Zhang W., Davidson E.J., Fingerlin T.E., Kechris K, & Dabelea D. (2018). Epigenetic marks of in utero exposure to gestational diabetes and childhood adiposity outcomes: the EPOCH study. Diabetic medicine : a journal of the British Diabetic Association, 35(5), 612-620.

Publication 2018

Pyromark Assay Design Software

Assay Bisulfite Primers

Corresponding Organization : Colorado School of Public Health

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Variable analysis

independent variables

Bisulfite-converted DNA

dependent variables

DNA methylation

control variables

PCR primers designed in the Pyromark Assay Design Software (Qiagen, Germantown, MD, USA)
PCR and sequencing primers listed in Table S1

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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