SDS–PAGE and Western blot analysis were performed exactly as described previously (Barbosa et al., 2015 (link)). Antibodies used were as follows. Primary antibodies were mouse anti-V5, (1:10,000; Life Technologies), rabbit anti–actin 20-36 (1:2500; Sigma-Aldrich), mouse anti–actin AC-40 (1:2500; Sigma-Aldrich), rabbit anti–γ-tubulin (1:2500; Sigma-Aldrich), anti–ApoA-IV 1D6B6 (1:2000; Cell Signaling, Leiden, Netherlands), rabbit phospho-GSK-3α/β(Ser-21/9) D17D2 (1:2000; Cell Signaling), and anti–CREB-H pS73 and pS81 (1:100; Barbosa et al., 2015 (link)). Secondary antibodies were conjugated with horseradish peroxidase (for detection by conventional chemiluminescence) or with Dylight680 or Dylight800 (Fisher Scientific, Lutterworth, United Kingdom) for detection by laser scanning using the LI-COR Odyssey Image system. The latter method was routinely employed for CREB-H detection using the phosphospecific antibodies because it allowed simultaneous analysis, on one blot, of total CREB-H species with the anti-mouse antibody to the epitope tag in one channel and the phosphorylated species with the anti-rabbit antibody to the phosphorylated peptide in a second channel.