Laser-induced Choroidal Neovascularization

CNV was induced as previously described (51 (link)). Briefly, mice were anesthetized and eyes were dilated and lasered with a slit lamp (Zeiss) mounted ophthalmic laser (IRIDEX). Each eye was treated with either 4 (CNV area quantification) or 8 (scRNA-Seq and flow cytometry analysis) focal laser burns (75 μm, 110 mW, 100 msec). Eyes with major bleeding at the time of laser were excluded. In Mac-depleting CD11c⁺Mac^iDTR mice, we i.p. injected 4 ng DT/g (MilliporeSigma, D0564, dissolved in PBS) at the time of laser injury and for every 2 days thereafter.

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Droho S., Rajesh A., Cuda C.M., Perlman H, & Lavine J.A. (2023). CD11c+ macrophages are proangiogenic and necessary for experimental choroidal neovascularization. JCI Insight, 8(7), e168142.

Publication 2023

D0564

Burns Flow cytometry Injury Mice Scrna seq Slit lamp

Corresponding Organization :

Other organizations : Northwestern University

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Variable analysis

independent variables

Laser treatment (4 or 8 focal laser burns)
Macrophage depletion (i.p. injection of DT in CD11c+MaciDTR mice)

dependent variables

CNV area quantification
ScRNA-Seq analysis
Flow cytometry analysis

control variables

Anesthesia
Pupil dilation
Slit lamp mounted ophthalmic laser (Zeiss)
Laser parameters (75 μm, 110 mW, 100 msec)
Exclusion of eyes with major bleeding

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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