Aequorin-based Ca2+ Imaging in Cells
Corresponding Organization : Montreal Neurological Institute and Hospital
Other organizations : MRC Mitochondrial Biology Unit, Medical Research Council, University of Cambridge, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, University of Toronto
Variable analysis
- Mitochondrial matrix-targeted (mtAEQmut) probe
- Wild-type cytosolic aequorin (CytAEQ) probe
- Cytosolic or mitochondrial Ca2+ concentration
- White 96-well plates (Corning)
- Adenovirus containing the probes
- Incubation conditions (37°C, 5% CO2)
- Washing and incubation buffers (BSS + Ca2+)
- Coelenterazine concentration (5 μM)
- Digitonin and CaCl2 concentrations (100 μM and 10 mM, respectively)
- Cells were digitonized and saturated with Ca2+ by injection of 100 μM digitonin and 10 mM CaCl2 to discharge all luminous potential
- Not explicitly mentioned
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