Transcriptomic Analysis of Microglia TCA/Glycolysis

The transcriptome analysis of the TCA/glycolytic genes of in vivo microglia was performed using our recently published data (Zhang et al., 2022)^{21 (link)}. In the study of Zhang et al., 2022 mice were given an intraperitoneal (ip) injection of 1 mg/kg LPS (Sigma‐Aldrich, Escherichia coli 011:B4,L4391) dissolved in DPBS (Lonza, BE17512F). Control mice received a respective volume of DPBS. After 3 h, animals were sacrificed under anesthesia and the brain was collected. Mouse microglia isolation from mouse were isolated from adult mouse brain using the protocol as described before⁶⁶. Briefly, microglia-enriched percoll fractions were sorted by gating the DAPI^negCD11b^highCD45^intLy6c^neg cells. 200.000-300.000 microglia per brain were sorted for RNAseq experiments.

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Sabogal-Guáqueta A.M., Marmolejo-Garza A., Trombetta-Lima M., Oun A., Hunneman J., Chen T., Koistinaho J., Lehtonen S., Kortholt A., Wolters J.C., Bakker B.M., Eggen B.J., Boddeke E, & Dolga A. (2023). Species-specific metabolic reprogramming in human and mouse microglia during inflammatory pathway induction. Nature Communications, 14, 6454.

Publication 2023

BE17512F

Adult Anesthesia Animals Brain Cells Escherichia coli Genes Glycolytic Intraperitoneal injection Isolation Microglia Mouse Percoll Transcriptome analysis

Corresponding Organization :

Other organizations : University of Groningen, University of Eastern Finland, University Medical Center Groningen

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Variable analysis

independent variables

Lipopolysaccharide (LPS) injection (1 mg/kg)

dependent variables

Transcriptome analysis of the TCA/glycolytic genes in in vivo microglia

control variables

Injection of DPBS (vehicle control)

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