AAV8-hSyn-Cre (titer: 6.5 × 10¹² molecules/ml) was sourced from the UNC Vector Core (Chapel Hill, NC, USA). AAVrg-CAG-GFP (titer: 5 × 10¹² vg/mL, this construct was a gift from Edward Boyden to Addgene- viral prep # 37825-AAVrg) and AAVrg pmSyn1-EBFP-Cre (titer: 6 × 10¹² vg/mL, this construct was a gift from Hongkui Zeng to Addgene -viral prep # 51507-AAVrg; Madisen et al., 2015 (link)), were sourced from Addgene (MA, USA). All constructs were administered 1 ul bilaterally and a minimum of 3 weeks incubation was allowed.
For CaMKIIα cell-specific knockdown and fear conditioning and forced swim studies, 0.1 ul of AAV9.CamKII.HI.eGFP-Cre.WPRE.SV40 (CaMKIIα Cre) [Penn Vector Core] (titer: 6.544 × 1013 diluted to 6.544 × 1011] was injected bilaterally and allowed 5 weeks to incubate.
For CaMKIIα cell-specific knockdown and fear conditioning and forced swim studies, 0.1 ul of AAV9.CamKII.HI.eGFP-Cre.WPRE.SV40 (CaMKIIα Cre) [Penn Vector Core] (titer: 6.544 × 1013 diluted to 6.544 × 1011] was injected bilaterally and allowed 5 weeks to incubate.
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