Assessing Channel Sensitivities in Mutant Oocytes

Oocyte and larval physiology were performed largely as described15 (link),28 (link), with additional details provided in Methods. Chemical sensitivities of wild type and mutant (dTRPA1-2C) channels were assessed by normalizing all currents to currents observed at 1mM AITC. Chemically unrelated insect repellents like DEET, IR-3535, and deltamethrin failed to activate dTRPA1 (K.K. and P.G., unpublished).

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Kang K., Pulver S.R., Panzano V.C., Chang E.C., Griffith L.C., Theobald D.L, & Garrity P.A. (2010). Analysis of Drosophila TRPA1 reveals an ancient origin for human chemical nociception. Nature, 464(7288), 597-600.

Publication 2010

Aitc Deet Deltamethrin Insect repellents Ir 3535 Larval Oocyte Physiology Sensitivities

Corresponding Organization :

Other organizations : Genomics (United Kingdom), Brandeis University

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Variable analysis

independent variables

Wild type and mutant (dTRPA1-2C) channels

dependent variables

Chemical sensitivities of wild type and mutant (dTRPA1-2C) channels
Currents observed at 1mM AITC

control variables

Oocyte and larval physiology procedures largely as described in references 15 and 28

controls

Currents observed at 1mM AITC used as a normalization control

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