Liver tissues were homogenized in cold mammalian cell lysis buffer with protease and phosphatase inhibitor cocktails as described previously (32 (link)). Tissue lysates were then centrifuged and the resulting supernatants were used for immunoblot analyses. Ago2 primary antibody (36 ) and α-Tubulin primary antibody (37 ) were purchased from Cell Signaling Technology. PPARα primary antibody (38 ) was purchased from Santa Cruz. Antimouse immunoglobulin G (IgG) secondary antibody (39 ) was purchased from GE Life Sciences. Horseradish peroxidase–conjugated goat antirabbit IgG (H+L) secondary antibody (40 ) was purchased from Invitrogen.
Protocol detail
Western Blot Analysis of Liver Tissue
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
α-Tubulin primary antibody
PPARα primary antibody
Horseradish peroxidase–conjugated goat antirabbit IgG (H+L) secondary antibody
Ago2
Antibody
Buffer
Cell
Cold
Goat
Horseradish peroxidase
Immunoblot analyses
Immunoglobulin g
Liver
Mammalian
Phosphatase
Protease
Tissue
α tubulin
Top 5 similar protocols
Variable analysis
independent variables
- None explicitly mentioned
- Protein expression levels of Ago2, α-Tubulin, and PPARα
- None explicitly mentioned
- Positive control: None mentioned
- Negative control: None mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!