Genetically Engineered Mouse Models for NLRP3 Studies

Casp1^−/− were kindly provided by Dr. Thirumala-Devi Kanneganti (St. Jude Children's Research Hospital). Cre-ER^TM (B6.Cg-Tg(CAG-cre/Esr1*)5Amc/J) mice and lysozyme M-Cre mice were purchased from The Jackson Laboratory (Sacramento, CA). Nlrp3^fl(D301N)/+ mice were kindly provided by Dr. Hal Hoffman (University of California, San Diego), and have been previously described (52 (link), 54 (link), 85 (link)). Nlrp3^CA/+ mice with constitutive activation of NLRP3 in myeloid cells driven by lysozyme M-Cre have been previously described (54 (link)). Cre-ER^TM mice and Nlrp3^fl(D301N)/+ mice were crossed to generate Nlrp3^fl(D301N)/+;Cre-ER^TM mice and Cre-ER^TM mice. Injection of tamoxifen (i.p., 75 mg/kg body weight; Sigma-Aldrich) to Nlrp3^fl(D301N)/+;Cre-ER^TM mice and Cre-ER^TM mice to yield inducible Nlrp3^CA (iNlrp3^CA) mice and control mice, respectively, has been previously described (85 (link)). Gsdmd^−/− mice were kindly provided by Dr. V. M. Dixit (Genentech, South San Francisco, CA). Asc-citrine and Gsdme^−/− mice were purchased from The Jackson Laboratory (Sacramento, CA). All mice were on the C57BL6J background and mouse genotyping was performed by PCR. All procedures were approved by the Institutional Animal Care and Use Committee of Washington University School of Medicine in St. Louis.