Fourier-domain OCT (FD-OCT) (RTVue 100, software version 6.1; Optovue Inc, Fremont, CA) and IVCM (ConfoScan 4; Nidek Technologies, Gamagori, Japan) of the central cornea were performed using the previously described methods at 2 separate sessions.23 (link) The tear meniscus was assessed using FD-OCT before sedation as previously described,24 (link) and then the dogs were intravenously sedated (≤6 years old: dexmedetomidine 2.5 μg/kg, buprenorphine: 0.01 mg/kg; >6 years old: acepromazine 0.01 mg/kg, buprenorphine 0.01 mg/kg) for FD-OCT and IVCM of the central cornea.25 (link) Keratocytes in the anterior and posterior stroma were counted as previously described.23 (link)
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Multimodal Corneal Imaging in Canines
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Corresponding Organization : University of California, Davis
Other organizations : University of Wisconsin–Madison
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Variable analysis
independent variables
- Sedation protocol for dogs (≤6 years old: dexmedetomidine 2.5 μg/kg, buprenorphine: 0.01 mg/kg; >6 years old: acepromazine 0.01 mg/kg, buprenorphine 0.01 mg/kg)
- Tear meniscus assessed using FD-OCT
- Central cornea characteristics assessed using FD-OCT and IVCM
- Keratocyte count in the anterior and posterior stroma
- Central cornea assessed at 2 separate sessions
- Tear meniscus assessed before sedation
- Methods used for FD-OCT and IVCM were the same as previously described
- No positive or negative controls were explicitly mentioned in the provided information.
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