Culturing Human Cancer Cell Lines

Human cancer cell lines MA-11, MDA-MB-231 (ATCC #HTB-26™), Hs578T (ATCC #HTB-126™), AU565 (ATCC #CRL-2351™), HCC1428 (ATCC #CRL-2327™), MCF7 (ATCC #HTB-22™), HCC1187 (ATCC #CRL-2322™), DU4475 (ATCC #HTB-123™), HCC1569 (ATCC #CRL-2330™), DLD1 (ATCC #CCL-221™), HPAF-II (ATCC #CRL-1997™) and LNCaP (ATCC #CRL-1740™), were maintained at 37°C in 5% CO₂ and cultured in either Dulbecco´s Modified Eagle's Medium (DMEM) or Roswell Park Memorial Institute Medium (RPMI) supplemented with 10% FBS.
All cancer cell lines, except MA-11, were obtained from the American Type Culture Collection (ATCC; www.atcc.org). The human MA-11 breast carcinoma cell line, established from bone marrow micrometastases of a patient with breast cancer [52 (link)], was obtained by Øystein Fodstad (Norwegian Radium Hospital, Oslo, Norway). Cell pellets and conditioned media from the Melmet 1 (MM1) and Melmet 5 (MM5) melanoma cell lines were a gift from Dr. Siri Tveito at the Norwegian Radium Hospital, Oslo, Norway, and were established from the biopsies of metastatic melanoma patients at the department of Tumor Biology, The Norwegian Radium Hospital Oslo, Norway [53 (link)].

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Fiskaa T., Knutsen E., Nikolaisen M.A., Jørgensen T.E., Johansen S.D., Perander M, & Seternes O.M. (2016). Distinct Small RNA Signatures in Extracellular Vesicles Derived from Breast Cancer Cell Lines. PLoS ONE, 11(8), e0161824.

Publication 2016

MDA-MB-231 Hs578T AU565 HCC1428 MCF7 HCC1187 DU4475 HCC1569 DLD1 HPAF-II LNCaP

Biopsies Bone marrow Breast cancer Breast carcinoma cell line Cancer Cell Cell lines Conditioned media Human Melanoma Patients Pellets Radium Tumor

Corresponding Organization : Nord University

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Variable analysis

independent variables

Cell lines (MA-11, MDA-MB-231, Hs578T, AU565, HCC1428, MCF7, HCC1187, DU4475, HCC1569, DLD1, HPAF-II, LNCaP, Melmet 1, Melmet 5)

dependent variables

Not explicitly mentioned

control variables

Culture conditions (37°C, 5% CO2)
Culture media (DMEM or RPMI, supplemented with 10% FBS)

controls

Positive control: None specified
Negative control: None specified

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