SPTs were performed with a panel of food and inhalant allergens prevalent in the region [9 (link)] (see S2 Appendix). These were provided by Inmunotek® (Madrid, Spain). Native Pru p 9 was obtained as described [13 (link)]. The ImmunoCAP® test (Thermo Fisher Scientific, Wahtham, Massachussets, USA) was used to detect SIgE to Pru p 1, Pru p 3, Pru p 4 and Pru p 7 with values higher than 0.35 kUA/L indicative of the presence of antibodies.
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