Chlorophyll a and chlorophyll b concentrations were quantified from 0.1 g of leaf tissue in ethanol extracts, with absorbance readings taken at 645 nm and 663 nm using a UVmini-1240 spectrophotometer (Shimadzu, Japan) (Wellburn, 1994 (link); Wei et al., 2022 (link)). The total chlorophyll content per unit area (Chlarea, mg m-2) was then calculated by multiplying chlorophyll a+b with the leaf mass per unit area (LMA) (n = 6).
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