Glomerular DNA Isolation and Characterization

Paramagnetic isolation of glomeruli from both genotypes was performed as described in a previous study (40 (link)). Genomic DNA was isolated from harvested glomeruli by the addition of Direct PCR Tail Peqgold (Peqlab) and proteinase K (Qiagen), according to the manufacturer’s protocol, and incubation for 3h at 55°C and 800 rpm. Subsequent inactivation of proteinase K was achieved by heating the sample to 85°C for 45 min. End point PCR using Taq DNA Polymerase (M0273X, New England Biolabs) and primers indicated by The Jackson Laboratories for the B6.Cg-Tg(NPHS2-cre)295^Lbh/J strain verified successful excision of the floxed transgene by Nphs2-driven Cre recombinase (Supplementary Figure 1D).