C2C12 Myocyte Differentiation Protocol

C2C12 cells were obtained from American Type Culture Collection (ATCC, CRL-1772™, Manassas, VA, USA). The cells were cultured in growth medium consisting of Dulbecco's modified eagle medium (DME-M), 10% heat-inactivated fetal calf serum (Biowest, St. Louis, MO), and 1% penicillin-streptomycin. C2C12 cells were successfully differentiated into myocytes or myotubes in a differentiation medium consisting of DMEM, 2% heat-inactivated horse serum (Invitrogen, Carlsbad, CA, USA) and 1% penicillin-streptomycin. All these cells were maintained at 37° C in a humidified atmosphere containing 5% CO2 [66 (link)].

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Wang Y., Zhao Z.J., Kang X.R., Bian T., Shen Z.M., Jiang Y., Sun B., Hu H.B, & Chen Y.S. (2020). lncRNA DLEU2 acts as a miR-181a sponge to regulate SEPP1 and inhibit skeletal muscle differentiation and regeneration. Aging (Albany NY), 12(23), 24033-24056.

Publication 2020

C2C12 cells DME-M fetal calf serum horse serum

2 heat Atmosphere Cells Eagle Fetal calf serum Horse Myocytes Myotubes Penicillin Serum Streptomycin

Corresponding Organization :

Other organizations : Jinshan Hospital of Fudan University, Shanghai Medical College of Fudan University, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai First People's Hospital

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Variable analysis

independent variables

Culture medium (growth medium vs. differentiation medium)

dependent variables

Differentiation of C2C12 cells into myocytes or myotubes

control variables

Temperature (37°C)
Atmosphere (5% CO2, humidified)
Cell line (C2C12 cells from ATCC)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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