gDNA Extraction from Plant Leaves

gDNA was extracted from young leaves of plant materials using a modified cetyltrimethylammonium bromide (CTAB) method (Han et al., 2018 (link)). Young leaf tissues were frozen in liquid nitrogen and finely ground using a 5 mm steel bead with a vortex mixer (DAIHAN Scientific, Wonju, Korea). The concentration and quality of extracted gDNA were measured with a Nanodrop spectrophotometer (BioTek, Winooski, VT, USA) and diluted to a final concentration of 20 ng/μL in triple distilled water (TDW).

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Yi S., Lee D.G., Back S., Hong J.P., Jang S., Han K, & Kang B.C. (2022). Genetic mapping revealed that the Pun2 gene in Capsicum chacoense encodes a putative aminotransferase. Frontiers in Plant Science, 13, 1039393.

Publication 2022

vortex mixer Nanodrop spectrophotometer

Cetyltrimethylammonium bromide Frozen Leaf Nitrogen Steel Tissues

Corresponding Organization : Seoul National University

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Variable analysis

independent variables

Extraction method (modified CTAB)

dependent variables

Concentration of extracted gDNA
Quality of extracted gDNA

control variables

Plant material (young leaves)
Grinding method (vortex mixer with steel bead)
Measurement of concentration and quality (Nanodrop spectrophotometer)
Dilution of extracted gDNA (20 ng/μL in TDW)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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