Flow Cytometric Analysis of Apoptosis

All the chromogen-conjugated antibodies used in flow cytometry and BD Trucount beads were purchased from BD Biosciences. Fluorescein isothiocyanate (FITC)-conjugated annexin V was purchased from Life Technologies. Goat F(ab′)₂ anti-human IgM was purchased from SouthernBiotech. Ibrutinib (PCI-32765) was purchased from Selleck Chemical(12 (link)).

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Boysen J., Nelson M., Magzoub G., Maiti G.P., Sinha S., Goswami M., Vesely S.K., Shanafelt T.D., Kay N.E, & Ghosh A.K. (2016). Dynamics of Microvesicle Generation in B Cell Chronic Lymphocytic Leukemia: Implication in Disease Progression. Leukemia, 31(2), 350-360.

Publication 2016

BD Trucount beads Fluorescein isothiocyanate (FITC)-conjugated annexin V Goat F(ab′)2 anti-human IgM Ibrutinib

Annexin v Anti igm Antibodies Chromogen Flow cytometry Fluorescein Goat Human Ibrutinib Isothiocyanate Pci 32765

Corresponding Organization :

Other organizations : Mayo Clinic in Arizona, University of Oklahoma Health Sciences Center

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Variable analysis

independent variables

Ibrutinib (PCI-32765)

dependent variables

Not explicitly mentioned

control variables

All the chromogen-conjugated antibodies used in flow cytometry and BD Trucount beads were purchased from BD Biosciences
Fluorescein isothiocyanate (FITC)-conjugated annexin V was purchased from Life Technologies
Goat F(ab′)2 anti-human IgM was purchased from SouthernBiotech

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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