Recombinant AMP-17 Protein Production

The recombinant protein AMP-17 was prepared according to the previous method [21 (link),23 (link)]. Briefly, a single positive colony of E. coli BL21 (DE3) containing plasmid pET-28a (+)—(AMP-17) was inoculated in LB liquid medium containing kanamycin and grown overnight by shaking. The following day, the above bacterial suspension was expanded at a ratio of 1:100 to logarithmic growth phase. Then, Isopropyl-beta-D-thiogalactopyranoside (IPTG, Solarbio, Beijing, China) was added to the bacterial suspension and grown at 32 °C for 24 h. Subsequently, the recombinant protein AMP-17 was obtained by ultrasonic fragmentation, inclusion body lysis, Ni-NTA column purification, and ultrafiltration.