Infrared Thermography of Adipocyte Heat Production

WT and Mstn^−/− SVF cells were seeded at the density of 10 000 cells per cm² in the center wells of 96-well-plates. Wells surrounding the plated SVF wells contained 200 μl phosphate-buffered saline each. Mature adipocytes were derived from SVF cells after white adipogenic differentiation, as described above. Heat production from WT and Mstn^−/− adipocytes was measured by infrared thermography. Infrared thermography was performed as previously described,^{27 (link)} with modifications. In brief, the 96-well-plates containing the cells were kept on a heating block maintained at 35 °C within an insulating box. Images were captured using an infrared camera (FLIR T420, Wilsonville, OR, USA) and then analyzed using the FLIR Tools software (FLIR). Data were collected from triplicate plates, five wells per genotype per plate.