Purification and Detection Reagents

Blasticidin hydrochloride (10 mg/ml stock solution), hygromycin B (50 mg/ml solution), Geneticin sulfate (75% [wt/vol] purity), SuperSignal West Pico Plus chemiluminescent substrate, 6×His tag monoclonal antibody horseradish peroxidase (His.H8), and mouse antihemagglutinin (anti-HA) monoclonal antibody were from Invitrogen (Thermo Fisher Scientific). l-Histidine, anti-α-tubulin monoclonal antibody, mouse anti-c-Myc, ammonia assay kit, acid-washed glass beads (425 to 600 μm), protease inhibitor cocktail (catalog no. P2815), resazurin, EDAC [1-ethyl-3-(dimethylaminopropyl)carbodiimide] HCl, and rabbit anti-FLAG were from Sigma Chemical Co., and chicken anti-GFP was from Abcam. Guinea pig anti-vacuolar proton pyrophosphatase (VP1) was produced in-house (30 (link)), rabbit anti-pyruvate phosphate dikinase (PPDK) was a gift from Frédéric Bringaud (University of Bordeaux, France), mouse anti-histidine ammonia lyase (HAL) was kindly provided by Ariel Silber (University of São Paulo, Brazil). Aminomethylenediphosphonate (AMDP) was a gift from Eric Oldfield (University of Illinois at Urbana-Champaign), polyP₁₀₀ (i.e., 100 P_i units) was kindly provided by Shiba Toshikazu (RegeneTiss Co., Japan) or was purchased from Kerafast. Polymethacrylate beads (ReliZyme EA113; particle size, S, EC-HA) carrying an ethylenediamino group were purchased from Resindion srl (Milan, Italy).