Visualization and Quantification of JUNV Infection
Corresponding Organization : Harvard University
Variable analysis
- CH223191 treatment
- Kynurenine treatment
- Percentage of NP-positive cells
- Fluorescence intensity of NP-positive cells
- Viral foci size
- Huh-7 monolayers
- JUNV infection
- Methanol fixation at 48 h post-infection
- Primary mouse monoclonal anti-NP antibodies (1:200)
- Secondary Alexa Fluor 488 conjugated anti-mouse goat antibodies (1:200)
- DAPI staining (1:1000)
- Olympus IX71 fluorescence microscope
- QImaging Exi-Aqua camera
- Fiji software (version 1.53v)
- No information about positive or negative controls provided.
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