Receptor Blockade in Neurotransmitter Studies

The following neurotransmitter/neuropeptide receptor blockers were used: CCK-A receptor antagonist devazepide (2 mg kg^–1 in 5% DMSO PBS; Sigma)^{14 (link),53 (link)}, cocktail of the ionotropic glutamate receptor antagonist KA (150 μg kg^–1 in PBS, stock made in 1 M NaOH then diluted, pH 7.4; Sigma)^{14 (link),54 (link)} and the metabotropic glutamate receptor antagonist AP3 (1 mg kg^–1 in PBS, stock made in 1 M NaOH diluted, pH 7.4; Sigma)^{14 (link),55 (link)} and non-selective P2-purinoreceptor antagonist PPADs (25 mg kg^–1 in PBS; Sigma)^{32 (link),56 (link)–58 (link)}. Following recording of a preinhibitor response, one inhibitor was delivered over 1 min (devazepide and PPADs were delivered at 10 μl g^–1; the KA/AP3 cocktail was delivered at 20 μl g^–1). Infusion of the selected sugar ligand was repeated for postinhibitor recording after an incubation period of 5–8 min for devazepide and 3–5 min for KA/AP3 and PPADs.

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Buchanan K.L., Rupprecht L.E., Kaelberer M.M., Sahasrabudhe A., Klein M.E., Villalobos J.A., Liu W.W., Yang A., Gelman J., Park S., Anikeeva P, & Bohórquez D.V. (2022). The preference for sugar over sweetener depends on a gut sensor cell. Nature Neuroscience, 25(2), 191-200.

Publication 2022

devazepide PPADs

Cck a receptor Devazepide Dmso Glutamate receptor antagonist Ionotropic glutamate receptor Ligand Metabotropic glutamate receptor Neuropeptide Neuropeptide receptor Neurotransmitter Neurotransmitter receptor P2 purinoreceptor Ppads Sugar

Corresponding Organization :

Other organizations : Duke University, Massachusetts Institute of Technology, McGovern Institute for Brain Research

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Variable analysis

independent variables

CCK-A receptor antagonist devazepide (2 mg kg–1 in 5% DMSO PBS; Sigma)
Cocktail of the ionotropic glutamate receptor antagonist KA (150 μg kg–1 in PBS, stock made in 1 M NaOH then diluted, pH 7.4; Sigma) and the metabotropic glutamate receptor antagonist AP3 (1 mg kg–1 in PBS, stock made in 1 M NaOH diluted, pH 7.4; Sigma)
Non-selective P2-purinoreceptor antagonist PPADs (25 mg kg–1 in PBS; Sigma)

dependent variables

Preinhibitor response
Postinhibitor response

control variables

PBS (vehicle for drug solutions)

Annotations

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