BDNF Expression in Mouse Brain

RNA isolation and reverse transcription reaction were performed as previously described in Kim et al. (2019 (link)). RNA extraction was done from p9 whole brain tissue of WT and BDNF^+/– mice using RNAqueous kit with no alterations to procedure (AM1931, Thermofisher). qPCR was executed using 7900HT Fast Real-Time PCR system (Applied Biosystems), data were analyzed using SDS v2.4 (Applied Biosystems). GAPDH was used as a reference housekeeping gene. Delta (Δ) CT (Mean_Gene- Mean_GAPDH) was utilized to calculate ΔΔCT (ΔCT_Pos-ΔCT_Neg), and data was normalized to WT (100%). Mouse primers used: BDNF Forward: 5’-TCGTTCCTTTCGAGTTAGCC, BDNF Reverse: 5’-TTGGTAAACGGCACAAAAC, GAPDH Forward: 5’-AGTATGACTCCACTCACGGCAA, and GAPDH Reverse: 5’-TCTCGCTCCTGGAAGATGGT.