Cardiac Transplantation and Immune Checkpoint Blockade

Hearts from BALB/c donors were transplanted into
8‐10‐week‐old male
Irf4^fl/flCd4‐Cre or
CD45.1⁺ congenic mice by a previously described method.^{8 (link)} Some recipient mice were ip
injected with 400 μg Rat IgG, or 200 μg anti‐PD‐L1
(clone 10F.9G2) plus 200 μg anti-CTLA‐4 (clone 9D9) mAbs
(Bio‐X‐Cell, West Lebanon, NH) on days 0, 3, 5 post‐heart
grafting.

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Zhang H., Wu J., Zou D., Xiao X., Yan H., Li X.C, & Chen W. (2018). Ablation of interferon regulatory factor 4 in T cells induces “memory” of transplant tolerance that is irreversible by immune checkpoint blockade. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 19(3), 884-893.

Publication 2018

anti‐PD‐L1(clone 10F.9G2)

Cell Clone Congenic mice Ctla 4 Donors Hearts Mabs Mice Pd l1

Corresponding Organization : Cornell University

Other organizations : Second Xiangya Hospital of Central South University, Central South University, Union Hospital, Huazhong University of Science and Technology

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Variable analysis

independent variables

Recipient mouse type: Irf4^fl/fl Cd4-Cre or CD45.1+ congenic mice
Injections: 400 μg Rat IgG, or 200 μg anti-PD-L1 (clone 10F.9G2) plus 200 μg anti-CTLA-4 (clone 9D9) mAbs

dependent variables

Heart graft survival

control variables

Age of recipient mice: 8-10 weeks old
Sex of recipient mice: Male
Donor heart source: BALB/c
Heart transplantation method: Previously described method (reference 8)

controls

Positive control: Recipient mice injected with Rat IgG
Negative control: Recipient mice not injected with any antibodies

Annotations

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