Cell Culture Protocols for HEK 293, Ocular Melanoma

HEK 293 Gα_q/11 KO cells were generously provided by Dr Asuka Inoue and were described previously (56 (link)). The HEK 293 Gα_q/11 KO cells were cultured in Dulbecco’s modified Eagle’s medium (Corning, Cat # 10-017-CV) with 10% fetal bovine serum (FBS) (Gemini, Cat # 900-108) and 1% penicillin/streptomycin (Sigma, Cat # P4333). HEK 293 Gβ₁γ₂ stable cells were described previously (35 (link)) and were supplemented with 10% FBS, 1% penicillin/streptomycin, and 0.5 mg/ml of G418 (Invivogen, Cat # ant-gn-5). OCM-3, 92.1, and OMM1.3 cells were provided by Dr Andrew Aplin and have been previously described (19 (link)). UM001 cells were also described previously and obtained from Dr Takami Sato (57 (link)). The Mel202 cell line was from Dr Bruce Ksander. The OCM-3, 92.1, Mel202, OMM1.3, and UM001 cells were cultured in RPMI 1640 with L-Glutamine and 25 mM Hepes (Corning, Cat # 10-041-CV) supplemented with 10% FBS. All cell culture plates were obtained from Costar, Fisher, or GenClone.