Complement System Analysis in Cystic Fibrosis

Plasma specimens were collected from healthy donors (n = 3) and pwCF with (n = 16) and without (n = 11) known NTM infection. The samples were analyzed using Human Complement Panel II (Millipore, Burlington, MA) in a Luminex Magpix. Analytes tested included C1q, C3, C3b/iC3b, C4, factor B, and factor H. Hierarchical clustering was performed using Morpheus analysis software (Broad Institute) with log₂-normalized values, using Euclidean distance metric and complete linkage.

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Lenhart-Pendergrass P.M., Malcolm K.C., Wheeler E., Rysavy N.M., Poch K., Caceres S., Calhoun K.M., Martiniano S.L, & Nick J.A. (2023). Deficient Complement Opsonization Impairs Mycobacterium avium Killing by Neutrophils in Cystic Fibrosis. Microbiology Spectrum, 11(1), e03279-22.

Publication 2023

Magpix

Donors Factor b Factor h Human Ic3b Infection Plasma

Corresponding Organization : University of California, San Diego

Other organizations : University of Colorado Denver, National Jewish Health

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Variable analysis

independent variables

Donor health status (healthy vs. pwCF)
Known NTM infection status in pwCF (with vs. without)

dependent variables

Levels of C1q
Levels of C3
Levels of C3b/iC3b
Levels of C4
Levels of factor B
Levels of factor H

control variables

Sample collection from healthy donors (n = 3)
Sample collection from pwCF (n = 16 with known NTM infection, n = 11 without known NTM infection)

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