Negative Stain EM Analysis of WT and Mutant PNGS Proteins
Corresponding Organization : Cornell University
Other organizations : Scripps Research Institute, University of Oxford, International AIDS Vaccine Initiative
Variable analysis
- WT protein
- NxT PNGS mutants
- RM20E1 complexes (SOSIP trimers incubated with 6-fold molar excess per protomer Fab at RT overnight)
- Purified complexes (analyzed by negative stain EM)
- Experimental procedures (sample preparation, imaging, and analysis) were the same as previously described (Cottrell et al., 2020)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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