To induce diabetes, mice received a single intraperitoneal (i.p.) dose of 195 mg/kg STZ (Sigma-Aldrich, St. Louis, MO) prepared in 5 M sodium citrate, pH 4.5, or vehicle (Ho et al. 2011 (link)). Blood glucose levels were measured 7, 10 and 20 days after STZ or vehicle injection using a portable Freestyle glucometer (Abbott Laboratories, Abbott Park, IL). Blood was obtained via tail snip. Mice with blood glucose values > 300 mg/dl were included in the STZ groups. Glucose levels were then measured on a weekly basis, in the morning between 08:00 – 10:00, until study completion.
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Streptozotocin-Induced Diabetes in Mice
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Corresponding Organization : University of Pennsylvania
Other organizations : Drexel University
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Variable analysis
independent variables
- Intraperitoneal (i.p.) injection of 195 mg/kg STZ (Sigma-Aldrich, St. Louis, MO) prepared in 5 M sodium citrate, pH 4.5
- Intraperitoneal (i.p.) injection of vehicle (5 M sodium citrate, pH 4.5)
- Blood glucose levels measured 7, 10, and 20 days after STZ or vehicle injection
- Weekly blood glucose levels measured in the morning between 08:00 – 10:00 until study completion
- Mice used in the study
- Portable Freestyle glucometer (Abbott Laboratories, Abbott Park, IL) used to measure blood glucose levels
- Blood obtained via tail snip
- Positive control: Mice receiving STZ injection
- Negative control: Mice receiving vehicle injection
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