ATPase activity assay of ATP1A3

COS-7 cells expressing wild-type or mutant alleles of ATP1A3 were assessed in the ATPase assay as described previously (4 (link)). Briefly, synthesized adenosine 5′-diphosphate (ADP) by ATPase reaction was detected using the EnzyLight ADP Assay Kit (BioAssay Systems, Hayward, CA, USA). ATPase activity was estimated by subtracting the luminescence signal after a 1-hour pretreatment with 100 μM ouabain (Sigma-Aldrich, St. Louis, MO, USA) from the luminescence reading without ouabain.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Miyatake S., Kato M., Kumamoto T., Hirose T., Koshimizu E., Matsui T., Takeuchi H., Doi H., Hamada K., Nakashima M., Sasaki K., Yamashita A., Takata A., Hamanaka K., Satoh M., Miyama T., Sonoda Y., Sasazuki M., Torisu H., Hara T., Sakai Y., Noguchi Y., Miura M., Nishimura Y., Nakamura K., Asai H., Hinokuma N., Miya F., Tsunoda T., Togawa M., Ikeda Y., Kimura N., Amemiya K., Horino A., Fukuoka M., Ikeda H., Merhav G., Ekhilevitch N., Miura M., Mizuguchi T., Miyake N., Suzuki A., Ohga S., Saitsu H., Takahashi H., Tanaka F., Ogata K., Ohtaka-Maruyama C, & Matsumoto N. (2021). De novo ATP1A3 variants cause polymicrogyria. Science Advances, 7(13), eabd2368.

Publication 2021

ouabain

Adenosine diphosphate Alleles Atp1a3 Atpase Bioassay Cos 7 cells Luminescence Ouabain

Corresponding Organization : Yokohama City University

Other organizations : Yokohama City University Hospital, Showa University, Tokyo Metropolitan Institute of Medical Science, Nara Institute of Science and Technology, Hamamatsu University School of Medicine, RIKEN Center for Brain Science, Kyushu University, Fukuoka Dental College, Fukuoka Children's Hospital and Medical Center for Infectious Diseases, Tottori University, Yamagata University, The University of Tokyo, Tokyo Medical and Dental University, RIKEN Center for Integrative Medical Sciences, Tottori Prefectural Central Hospital, Otsu Red Cross Hospital, Osaka Hospital, National Epilepsy Center, Rambam Health Care Campus, Nagaoka Red Cross Hospital

Top 5 similar protocols

Variable analysis

independent variables

Wild-type alleles of ATP1A3
Mutant alleles of ATP1A3

dependent variables

ATPase activity

control variables

Pretreatment with 100 μM ouabain

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!