In the initial approach, a targeted metabolomics approach using Waters’ ACQUITY UPLC® System with Xevo triple-quadrupole mass spectrometer (Waters, MA, USA) combined with the commercially available AbsoluteIDQ® p180 Kit (Biocrates Life Sciences AG, Austria) was used for rapid and quantitative analyses of 40 acylcarnitines according to the manufacturer’s protocol. The method combines the extraction of analytes with stable isotope dilution mass spectrometric analysis. The AbsoluteIDQ p180 Kit contains a 96-deep-well plate with a filter plate attached with sealing tape, as well as isotope-labeled internal standards and seven calibrators sufficient for quantitation of metabolites and three different quality control samples (low, medium and high spiked human plasma). The assay procedure has been previously described elsewhere [25 (link)–27 (link)].
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Targeted Metabolomics for Acylcarnitine Quantification
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Corresponding Organization :
Other organizations : University of Arkansas for Medical Sciences, Arkansas Children's Hospital, Medical College of Wisconsin, National Center for Toxicological Research, United States Food and Drug Administration, Kosair Charities, Kosair Children's Hospital, University of Louisville, Children's Mercy Hospital, Akron Children's Hospital, Northeast Ohio Medical University, Cook Children's Health Care System, Children's National
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Variable analysis
independent variables
- Not explicitly mentioned
- Levels of 40 acylcarnitines
- Isotope-labeled internal standards
- Seven calibrators for quantitation of metabolites
- Three different quality control samples (low, medium and high spiked human plasma)
- Positive controls: Seven calibrators for quantitation of metabolites
- Negative controls: Three different quality control samples (low, medium and high spiked human plasma)
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